More or less-On the influence of labelling strategies to infer cell population dynamics.

ABSTRACT: The adoptive transfer of labelled cell populations has been an essential tool to determine and quantify cellular dynamics. The experimental methods to label and track cells over time range from fluorescent dyes over congenic markers towards single-cell labelling techniques, such as genetic barcodes. While these methods have been widely used to quantify cell differentiation and division dynamics, the extent to which the applied labelling strategy actually affects the quantification of the dynamics has not been determined so far. This is especially important in situations where measurements can only be obtained at a single time point, as e.g. due to organ harvest. To this end, we studied the appropriateness of various labelling strategies as characterised by the number of different labels an

SUBMITTER: Gabel M 

PROVIDER: S-EPMC5646766 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature