Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array.

ABSTRACT: Protein array technology not only identifies a large number of proteins but also determines their expression levels. In the present study, antibody array analysis is used to decipher the proteins involved in hesperidin-induced cell death in HepG2 cells. Altered proteins in hesperidin treated cells were compared with that of untreated control cells by using a RayBio® Label‑based (L series) human antibody array kit. The identified proteins were further confirmed using western blot analysis. STRING software based analysis was used to determine the protein‑protein interactions. Many proteins related to signal transduction, cellular mechanisms, cell growth and proliferation regulatory proteins were identified. Among the proteins identified Hsp90, Smac/DIABLO, Prdx6 and FRK were significantly re

SUBMITTER: Yumnam S 

PROVIDER: S-EPMC5647072 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature