OCT4 impedes cell fate redirection by the melanocyte lineage master regulator MITF in mouse ESCs.

ABSTRACT: Ectopic expression of lineage master regulators induces transdifferentiation. Whether cell fate transitions can be induced during various developmental stages has not been systemically examined. Here we discover that amongst different developmental stages, mouse embryonic stem cells (mESCs) are resistant to cell fate conversion induced by the melanocyte lineage master regulator MITF. By generating a transgenic system we exhibit that in mESCs, the pluripotency master regulator Oct4, counteracts pro-differentiation induced by Mitf by physical interference with MITF transcriptional activity. We further demonstrate that mESCs must be released from Oct4-maintained pluripotency prior to ectopically induced differentiation. Moreover, Oct4 induction in various differentiated cells represses their

SUBMITTER: Sheinboim D 

PROVIDER: S-EPMC5647326 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature