Crosstalk between proteins expression and lysine acetylation in response to patulin stress in Rhodotorula mucilaginosa.

ABSTRACT: The proteomic and lysine acetylation (Kac) changes, accompanying degradation of patulin in Rhodotorula mucilaginosa were analyzed using tandem mass tagging and N6-acetyllysine affinity enrichment followed by LC-MS/MS. Proteomic results showed that expression level of short-chain reductase protein and glutathione S-transferase involved in detoxification was significantly up-regulated. In addition, the expression levels of zinc-binding oxidoreductase and quinone oxidoreductase that are involved in antioxidant process, ABC transport and MFS transport responsible for chemical transport were activated when treated with patulin. The quantitative real time PCR (qRT-PCR) result also indicated these genes expression levels were increased when treated with patulin. Kac changes accompanying degradati

SUBMITTER: Zheng X 

PROVIDER: S-EPMC5647337 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature