The histone variant H3.3 G34W substitution in giant cell tumor of the bone link chromatin and RNA processing.

ABSTRACT: While transcription as regulated by histones and their post-translational modifications has been well described, the function of histone variants in this process remains poorly characterized. Potentially important insight into this process pertain to the frequently occurring mutations of H3.3, leading to G34 substitutions in childhood glioblastoma and giant cell tumor of the bone (GCTB). In this study, we have established primary cell lines from GCTB patients and used them to uncover the influence of H3.3 G34W substitutions on cellular growth behavior, gene expression, and chromatin compaction. Primary cell lines with H3.3 G34W showed increased colony formation, infiltration and proliferation, known hallmarks of tumor development. Isogenic cell lines with H3.3 G34W recapitulated the increa

SUBMITTER: Lim J 

PROVIDER: S-EPMC5647428 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature