Lower airways inflammation in patients with ARDS measured using endotracheal aspirates: a pilot study.

ABSTRACT:

Introduction

Our knowledge of acute respiratory distress syndrome (ARDS) pathogenesis is incomplete. The goal of this pilot study is to investigate the feasibility of measuring lower airways inflammation in patients with ARDS using repeated endotracheal aspirates (ETAs).

Methods

ETAs were obtained within 24 hours by intensive care unit admission from 25 mechanically ventilated patients with ARDS and 10 of them underwent a second ETA within 96 hours after the first sampling. In each sample, cell viability was assessed using trypan blue exclusion method and the total and differential cell counts were measured using Neubauer-improved cell counting chamber and cytospins stained with Diff-Quik.

Results

The median cell viability was 89 (IQR 80-93)%, with a median total cell

SUBMITTER: Spadaro S 

PROVIDER: S-EPMC5647481 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature