Probing Cell Adhesion Profiles with a Microscale Adhesive Choice Assay.

ABSTRACT: In this work, we introduce, to our knowledge, a new set of adhesion-based biomarkers for characterizing mammalian cells. Mammalian cell adhesion to the extracellular matrix influences numerous physiological processes. Current in vitro methods to probe adhesion focus on adhesive force to a single surface, which can investigate only a subcomponent of the adhesive, motility, and polarization cues responsible for adhesion in the 3D tissue environment. Here, we demonstrate a method to quantify the transhesive properties of cells that relies on the microscale juxtaposition of two extracellular matrix-coated surfaces. By multiplexing this approach, we investigate the unique transhesive profiles for breast cancer cells that are adapted to colonize different metastatic sites. We find that malignant

SUBMITTER: Kittur H 

PROVIDER: S-EPMC5647542 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature