The Detection of Nanoscale Membrane Bending with Polarized Localization Microscopy.

ABSTRACT: The curvature of biological membranes at the nanometer scale is critically important for vesicle trafficking, organelle morphology, and disease propagation. The initiation of membrane bending occurs at a length scale that is irresolvable by most superresolution optical microscopy methods. Here, we report the development of polarized localization microscopy (PLM), a pointillist optical imaging technique for the detection of nanoscale membrane curvature in correlation with single-molecule dynamics and molecular sorting. PLM combines polarized total internal reflection fluorescence microscopy and single-molecule localization microscopy to reveal membrane orientation with subdiffraction-limited resolution without reducing localization precision by point spread function manipulation. Membrane c

SUBMITTER: Kabbani AM 

PROVIDER: S-EPMC5647545 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature