Monitoring Microbial Mineralization Using Reverse Stable Isotope Labeling Analysis by Mid-Infrared Laser Spectroscopy.

ABSTRACT: Assessing the biodegradation of organic compounds is a frequent question in environmental science. Here, we present a sensitive, inexpensive, and simple approach to monitor microbial mineralization using reverse stable isotope labeling analysis (RIL) of dissolved inorganic carbon (DIC). The medium for the biodegradation assay contains regular organic compounds and ¹³C-labeled DIC with ¹³C atom fractions (x(¹³C)_DIC) higher than natural abundance (typically 2-50%). The produced CO₂ (x(¹³C) ≈ 1.11%) gradually dilutes the initial x(¹³C)_DIC allowing to quantify microbial mineralization using mass-balance calculations. For ¹³C-enriched CO₂ samples, a newly developed isotope ratio mid-inf

SUBMITTER: Dong X 

PROVIDER: S-EPMC5647565 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature