Nanoscale Membrane Budding Induced by CTxB and Detected via Polarized Localization Microscopy.

ABSTRACT: For endocytosis and exocytosis, membranes transition among planar, budding, and vesicular topographies through nanoscale reorganization of lipids, proteins, and carbohydrates. However, prior attempts to understand the initial stages of nanoscale bending have been limited by experimental resolution. Through the implementation of polarized localization microscopy, this article reports the inherent membrane bending capability of cholera toxin subunit B (CTxB) in quasi-one-component-supported lipid bilayers. Membrane buds were first detected with <50 nm radius, grew to >200 nm radius, and extended into longer tubules with dependence on the membrane tension and CTxB concentration. Compared to the concentration of the planar-supported lipid bilayers, CTxB was (12 ± 4)× more concentrated on the p

SUBMITTER: Kabbani AM 

PROVIDER: S-EPMC5647570 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature