Metastability Gap in the Phase Diagram of Monoclonal IgG Antibody.

ABSTRACT: Crystallization of IgG antibodies has important applications in the fields of structural biology, biotechnology, and biopharmaceutics. However, a rational approach to crystallize antibodies is still lacking. In this work, we report a method to estimate the solubility of antibodies at various temperatures. We experimentally determined the full phase diagram of an IgG antibody. Using the full diagram, we examined the metastability gaps, i.e., the distance between the crystal solubility line and the liquid-liquid coexistence curve, of IgG antibodies. By comparing our results to the partial phase diagrams of other IgGs reported in literature, we found that IgG antibodies have similar metastability gaps. Thereby, we present an equation with two phenomenological parameters to predict the approxi

SUBMITTER: Rowe JB 

PROVIDER: S-EPMC5647622 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature