An Electrochemical Aptasensor Using Coaxial Capillary with Magnetic Nanoparticle, Urease Catalysis and PCB Electrode for Rapid and Sensitive Detection of Escherichia coli O157:H7.

ABSTRACT: The continuous outbreaks of foodborne diseases have drawn public attentions to food safety. Early screening of foodborne pathogens is crucial to prevent and control of foodborne diseases. In this study, a novel electrochemical aptasensor was developed for rapid and sensitive detection of E. coli O157:H7 using the coaxial capillary with immune magnetic nanoparticles (MNPs) for specific separation of the target bacteria, the urease with urea for amplification of the impedance signals, and the PCB gold electrode for measurement of the impedance change. The streptavidin modified MNPs were conjugated with the biotinylated polyclonal antibodies (PAbs) to form the immune MNPs, and captured in the coaxial capillary with the line-up high gradient magnetic fields to separate the bacteria from

SUBMITTER: Wang L 

PROVIDER: S-EPMC5647763 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature