Fluorescent Protein-Based Turn-On Probe through a General Protection-Deprotection Design Strategy.

ABSTRACT: We demonstrated a general protection-deprotection strategy for the design of fluorescent protein biosensors through the construction of a turn-on Hg²⁺ sensor. A combination of fluorescent protein engineering and unnatural amino acid mutagenesis was used. Unlike previously reported fluorescent protein-based Hg²⁺ sensors that relied on the binding of Hg²⁺ to the sulfhydryl group of cysteine residues, a well-established chemical reaction, oxymercuration, was transformed into biological format and incorporated into our sensor design. This novel Hg²⁺ sensor displayed good sensitivity and selectivity both in vitro and in live bacterial cells. Over 60-fold change in fluorescence signal output was observed in the presence of 10 μM Hg²⁺, while

SUBMITTER: Shang X 

PROVIDER: S-EPMC5648012 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature