Isothermal diagnostic assays for the detection of soil-transmitted helminths based on the SmartAmp2 method.

ABSTRACT:

Background

Diagnosis of soil-transmitted helminths (STHs) has traditionally relied on stool microscopy, which has a number of critical deficiencies. Molecular diagnostics are powerful tools to identify closely related species, but the requirement for costly equipment makes their implementation difficult in low-resource or field settings. Rapid, sensitive and cost-effective diagnostic tools are crucial for accurate estimation of STH infection intensity in MDA programmes in which the goal is to reduce morbidity following repeated rounds of chemotherapy.

Results

In this study, colourimetric isothermal assays were developed using SmartAmp2 primer sets and reagents in loop-mediated amplification (LAMP) assays. Species-specific primer sets, designed on a specific target sequence i

SUBMITTER: Rashwan N 

PROVIDER: S-EPMC5648480 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature