Cellular and molecular characterization of gametogenic progression in ex vivo cultured prepuberal mouse testes.

ABSTRACT:

Background

Recently, an effective testis culture method using a gas-liquid interphase, capable of differentiate male germ cells from neonatal spermatogonia to spermatozoa has been developed. Nevertheless, this methodology needs deep analyses that allow future experimental approaches in basic, pathologic and/or reprotoxicologic studies. Because of this, we characterized at cellular and molecular levels the entire in vitro spermatogenic progression, in order to understand and evaluate the characteristics that define the spermatogenic process in ex vivo cultured testes compared to the in vivo development.

Methods

Testicular explants of CD1 mice aged 6 and 10 days post-partum were respectively cultured during 55 and 89 days. Cytological and molecular approaches were performed, a

SUBMITTER: Isoler-Alcaraz J 

PROVIDER: S-EPMC5648490 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature