Cortisol inhibits mTOR signaling in avascular necrosis of the femoral head.

ABSTRACT:

Background

ANFH is a major health problem, to which long lasting and definitive treatments are lacking. The aim of this study is to study RNA alterations attributed to cortisol-induced ANFH.

Methods

Rat models were stratified into three groups: in vitro group (n = 20) for molecular biological assays, control group (n = 3), and ANFH group induced using lipopolysaccharide and dexamethasone (n = 3). Bone marrow-derived endothelial progenitor cells (BM-EPCs) were extracted from the rats. An RNA expression array was performed on BM-EPCs, and enriched genes were subject to pathway analysis. In vitro studies following findings of array results were also performed using the isolated BM-EPCs.

Results

Significant alterations in mammalian target of rapamycin (mTOR) and HIF signa

SUBMITTER: Liao Y 

PROVIDER: S-EPMC5648506 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature