Unique N-terminal sequences in two Runx1 isoforms are dispensable for Runx1 function.

ABSTRACT:

Background

The Runt-related transcription factors (Runx) are a family of evolutionarily conserved transcriptional regulators that play multiple roles in the developmental control of various cell types. Among the three mammalian Runx proteins, Runx1 is essential for definitive hematopoiesis and its dysfunction leads to human leukemogenesis. There are two promoters, distal (P1) and proximal (P2), in the Runx1 gene, which produce two Runx1 isoforms with distinct N-terminal amino acid sequences, P1-Runx1 and P2-Runx1. However, it remains unclear whether P2-Runx specific N-terminal sequence have any specific function for Runx1 protein.

Results

To address the function of the P2-Runx1 isoform, we established novel mutant mouse models in which the translational initiation AUG (+1) c

SUBMITTER: Nieke S 

PROVIDER: S-EPMC5648507 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature