Augmenting Endogenous Levels of Retinal Annexin A1 Suppresses Uveitis in Mice.

ABSTRACT:

Purpose

The purpose of this study was to examine the expression of the anti-inflammatory protein Annexin A1 (AnxA1) in mice and human retinae during uveitis and to determine whether local administration of human recombinant AnxA1 (hrAnxA1) can suppress uveitis in mice.

Methods

Retinal sections from mice (healthy normal and uveitis) and postmortem human (no history of eye disease (n = 5) and uveitis (n = 7)) were stained for AnxA1 expression and imaged by immunofluorescence microscopy. AnxA1 cellular expression was determined by colabeling with CD45, glial fibrillary acidic protein (GFAP), and Iba-1 cells, with additional staining of AnxA1 receptors formyl peptide receptor 1 (FPR1) and FPRL1/FPR2. Mice with acute endotoxin-induced uveitis and chronic experimenta

SUBMITTER: Gardner PJ 

PROVIDER: S-EPMC5648521 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature