Dynamics of B Cell Recovery In Kidney/Bone Marrow Transplant Recipients.

ABSTRACT:

Background

Previous studies identified B cell gene signatures and predominance of specific B cell subsets as a marker of operational tolerance after kidney transplantation. These findings suggested a role for B cells in the establishment or maintenance of tolerance. Here we analyzed B cell recovery in 4 subjects, 3 of whom achieved tolerance after combined kidney/bone marrow transplantation.

Methods

Peripheral B cell subsets were examined longitudinally by flow cytometry. Immunoglobulin heavy chain repertoire analysis was performed using next-generation sequencing. Lastly, the patients' serum reactivity to HLA was assessed by Luminex.

Results

B cell counts recovered approximately 1 year posttransplant except for 1 subject who experienced delayed reconstitution. This s

SUBMITTER: Gao B 

PROVIDER: S-EPMC5648631 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature