Clostridium difficile PCR Cycle Threshold Predicts Free Toxin.

ABSTRACT: There is no stand-alone Clostridium difficile diagnostic that can sensitively and rapidly detect fecal free toxins. We investigated the performance of the C. difficile PCR cycle threshold (C_T ) for predicting free toxin status. Consecutive stool samples (n = 312) positive for toxigenic C. difficile by the GeneXpert C. difficile/Epi tcdB PCR assay were tested with the rapid membrane C. Diff Quik Chek Complete immunoassay (RMEIA). RMEIA toxin-negative samples were tested with the cell cytotoxicity neutralization assay (CCNA) and tgcBIOMICS enzyme-linked immunosorbent assay (ELISA). Using RMEIA alone or in combination with CCNA and/or ELISA as the reference method, the accuracy of C_T was measured at different C

SUBMITTER: Senchyna F 

PROVIDER: S-EPMC5648702 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature