Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles.

ABSTRACT: Stimulated emission depletion microscopy provides a powerful sub-diffraction imaging modality for life science studies. Conventionally, stimulated emission depletion requires a relatively high light intensity to obtain an adequate depletion efficiency through only light-matter interaction. Here we show efficient emission depletion for a class of lanthanide-doped upconversion nanoparticles with the assistance of interionic cross relaxation, which significantly lowers the laser intensity requirements of optical depletion. We demonstrate two-color super-resolution imaging using upconversion nanoparticles (resolution ~ 66 nm) with a single pair of excitation/depletion beams. In addition, we show super-resolution imaging of immunostained cytoskeleton structures of fixed cells (resolution ~ 82 n

SUBMITTER: Zhan Q 

PROVIDER: S-EPMC5648820 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature