Reference genes validation in Phenacoccus solenopsis under various biotic and abiotic stress conditions.

ABSTRACT: Real-time PCR (RT-qPCR) expression analysis is a powerful analytical technique, but for normalization of data requires the use of stable reference genes. However, suitable reference genes are still not known in the case of Phenacoccus solenopsis under variable experimental treatments. The present study focused on the identification of stable housekeeping genes as a reference for analysis under different abiotic and biotic factors in P. solenopsis. We analyzed the relative expression of six commonly used candidate reference genes in different developmental stages, host-feeding assay, temperature treatments and field distribution conditions. Expression stabilities were analyzed by geNorm, NormFinder, and RefFinder. Under developmental and field distribution conditions, β-Tubulin was found to

SUBMITTER: Arya SK 

PROVIDER: S-EPMC5648885 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature