The efficacy and further functional advantages of random-base molecular barcodes for absolute and digital quantification of nucleic acid molecules.

ABSTRACT: Accurate quantification of biomolecules in system-wide measurements is in high demand, especially for systems with limited sample amounts such as single cells. Because of this, digital quantification of nucleic acid molecules using molecular barcodes has been developed, making, e.g., transcriptome analysis highly reproducible and quantitative. This counting scheme was shown to work using sequence-restricted barcodes, and non-sequence-restricted (random-base) barcodes that may provide a much higher dynamic range at significantly lower cost have been widely used. However, the efficacy of random-base barcodes is significantly affected by base changes due to amplification and/or sequencing errors and has not been investigated experimentally or quantitatively. Here, we show experimentally that

SUBMITTER: Ogawa T 

PROVIDER: S-EPMC5648891 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature