Identification of Staphylococcus aureus Factors Required for Pathogenicity and Growth in Human Blood.

ABSTRACT: Staphylococcus aureus is a human commensal but also has devastating potential as an opportunistic pathogen. S. aureus bacteremia is often associated with an adverse outcome. To identify potential targets for novel control approaches, we have identified S. aureus components that are required for growth in human blood. An ordered transposon mutant library was screened, and 9 genes involved specifically in hemolysis or growth on human blood agar were identified by comparing the mutants to the parental strain. Three genes (purA, purB, and pabA) were subsequently found to be required for pathogenesis in the zebrafish embryo infection model. The pabA growth defect was specific to the red blood cell component of human blood, showing no difference f

SUBMITTER: Connolly J 

PROVIDER: S-EPMC5649012 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature