Neuronal Culture Microenvironments Determine Preferences in Bioenergetic Pathway Use.

ABSTRACT: In the brain, metabolic supply and demand is directly coupled to neuronal activation. Methods for culturing primary rodent brain cells have come of age and are geared toward sophisticated modeling of human brain physiology and pathology. However, the impact of the culture microenvironment on neuronal function is rarely considered. Therefore, we investigated the role of different neuronal culture supplements for neuronal survival and metabolic activity in a model of metabolic deprivation of neurons using oxygen deprivation, glucose deprivation, as well as live cell metabolic flux analysis. We demonstrate the impact of neuronal culture conditions on metabolic function and neuronal survival under conditions of metabolic stress. In particular, we find that the common neuronal cell culture supp

SUBMITTER: Sunwoldt J 

PROVIDER: S-EPMC5649214 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature