A novel prothrombin time method to measure all non-vitamin K-dependent oral anticoagulants (NOACs).

ABSTRACT:

Background

There is a clinical need for point-of-care (POC) methods for non-vitamin K-dependent oral anticoagulants (NOACs). We modified a routine POC procedure: Zafena's Simple Simon™ PT-INR, a room-temperature, wet-chemistry prothrombin time method of the Owren-type.

Methods

To either increase or decrease NOAC interference, two assay variants were devised by replacing the standard 10 µL end-to-end capillary used to add the citrated plasma sample to 200 µL of prothrombin time (PT) reagent by either a 20 µL or a 5 µL capillary. All assay variants were calibrated to show correct PT results in plasma samples from healthy and warfarin-treated persons.

Results

For plasmas spiked with dabigatran, apixaban, or rivaroxaban, the 20 µL variant showed markedly higher PT results

SUBMITTER: Lindahl TL 

PROVIDER: S-EPMC5649322 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature