Post-injury bladder management strategy influences lower urinary tract dysfunction in the mouse model of spinal cord injury.

ABSTRACT:

Aims

To examine the effects of a different number of daily bladder squeezes on bladder dysfunction in mice with spinal cord injury (SCI).

Methods

Spinal cord was transected at the Th8/9 in female C57BL/6N mice. Their bladders were manually squeezed to eliminate urine inside every day for 4 weeks. The mice were divided into three groups depending on the number of bladder squeezes; A: once daily, B: twice daily, C: three times daily. Four weeks after transection, single-filling cystometry were performed under an awake condition. NGF in the bladder mucosa and mRNA expression of P2X receptors and TRP channels in L6/S1 dorsal root ganglia (DRG) were measured.

Results

Bladder weight in group C was less than that of group A. Bladder capacity, post-void residual, and the numb

SUBMITTER: Wada N 

PROVIDER: S-EPMC5650194 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature