Significance of B10 cell in patients with thymoma complicated with myasthenia gravis.

ABSTRACT: A subset of regulatory B cells in humans has been identified as B10 cell which has the function of secreting interleukin-10. We evaluated the significance of B10 cell in patients with thymoma complicated with myasthenia gravis. In this study, 156 patients diagnosed with thymoma were enrolled, FCM was used to detected the percentage of Breg/CD19⁺B cells and CD19⁺B cells/PBMC, ELISA to evaluate the serum concentration of the relevant immunological markers; purified CD19⁺B cells in tissues by MACS; gene and protein expressions of CD19 and IL-10 by Real-time PCR and Western-Blot; double immunofluorescence staining to detect the distribution of CD19 and IL-10 in thymus tissues. Thymoma patients without MG mainly display the types A and AB of thymoma, whereas the

SUBMITTER: Lu Y 

PROVIDER: S-EPMC5650299 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature