Immune Repertoire after Immunization As Seen by Next-Generation Sequencing and Proteomics.

ABSTRACT: The immune system produces a diverse repertoire of immunoglobulins in response to foreign antigens. During B-cell development, VDJ recombination and somatic mutations generate diversity, whereas selection processes remove it. Using both proteomic and NGS approaches, we characterized the immune repertoires in groups of rats after immunization with purified antigens. Proteomics and NGS data on the repertoire are in qualitative agreement, but did show quantitative differences that may relate to differences between the biological niches that were sampled for these approaches. Both methods contributed complementary information in the characterization of the immune repertoire. It was found that the immune repertoires resulting from each antigen had many similarities that allowed samples to clust

SUBMITTER: VanDuijn MM 

PROVIDER: S-EPMC5650670 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature