The Copper Homeostasis Transcription Factor CopR Is Involved in H₂O₂ Stress in Lactobacillus plantarum CAUH2.

ABSTRACT: Transcriptional factors (TFs) play important roles in the responses to oxidative, acid, and other environmental stresses in Gram-positive bacteria, but the regulatory mechanism of TFs involved in oxidative stress remains unknown in lactic acid bacteria. In the present work, homologous overexpression strains with 43 TFs were constructed in the Lactobacillus plantarum CAUH2 parent strain. The strain overexpressing CopR displayed the highest sensitivity and a 110-fold decrease in survival rate under H₂O₂ challenge. The importance of CopR in the response to H₂O₂ stress was further confirmed by a 10.8-fold increase in the survival of a copR insertion mutant. In silico analysis of the genes flanking copR revealed putative Co

SUBMITTER: Yang Y 

PROVIDER: S-EPMC5651008 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature