Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.

ABSTRACT: Condensin protein complexes coordinate the formation of mitotic chromosomes and thereby ensure the successful segregation of replicated genomes. Insights into how condensin complexes bind to chromosomes and alter their topology are essential for understanding the molecular principles behind the large-scale chromatin rearrangements that take place during cell divisions. Here, we identify a direct DNA-binding site in the eukaryotic condensin complex, which is formed by its Ycg1^Cnd3 HEAT-repeat and Brn1^Cnd2 kleisin subunits. DNA co-crystal structures reveal a conserved, positively charged groove that accommodates the DNA double helix. A peptide loop of the kleisin subunit encircles the bound DNA and, like a safety belt, prevents its dissociation. Firm closure of the klei

SUBMITTER: Kschonsak M 

PROVIDER: S-EPMC5651216 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature