Activation of Wnt signaling reduces high-glucose mediated damages on skin fibroblast cells.

ABSTRACT: OBJECTIVES:High-glucose (HG) stress, a mimic of diabetes mellitus (DM) in culture cells, alters expression of a large number of genes including Wnt and NF-κB signaling-related genes; however, the role of Wnt signaling during HG-mediated fibroblast damage and the relationship between Wnt and NF-κB signaling have not been understood. In this study, we aimed to investigate the ffects of Wnt signaling on HG-mediated damages. MATERIALS AND METHODS:Wnt3a was treated to HG-stressed human primary foreskin fibroblasts and the levels of Wnt signaling markers and cell proliferation were monitored. In addition, Wnt3a and NF-κB signaling inhibitor were assisted to analyze the relationship between two pathways. RESULTS:The results indicated that HG treatment repressed β-catenin level, and Wnt3a treatmen

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5651481 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature