Proteomic analysis of trochophore and veliger larvae development in the small abalone Haliotis diversicolor.

ABSTRACT:

Background

Haliotis diversicolor is commercially important species. The trochophore and veliger are distinct larval stages in gastropod development. Their development involves complex morphological and physiological changes. We studied protein changes during the embryonic development of H. diversicolor using two dimensional electrophoresis (2-DE) and label-free methods, tandem mass spectrometry (MS/ MS), and Mascot for protein identification.

Results

A total of 150 2-DE gel spots were identified. Protein spots showed upregulation of 15 proteins and downregulation of 28 proteins as H. diversicolor developed from trochophore to veliger larvae. Trochophore and veliger larvae were compared using a label-free quantitative proteomic approach. A total of 526 proteins were identifie

SUBMITTER: Di G 

PROVIDER: S-EPMC5651566 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature