Expression of Luteinizing Hormone (LH) Subunit Genes in Mouse Testis.

ABSTRACT: Gonadotropins are heterodimers consisting an alpha chain (Cgα) and a beta chain. Interestingly, presence of complicated LH-β transcripts in rat testis was accidently found; testicular LH-β transcripts were confined in seminiferous tubules to spermatids, and the translated products were localized in the elongated spermatids. We hypothesized that mouse testis has potential to produce the tissue specific LH-β with similar structure to the rat testicular forms. To verify our hypothesis, we examined the adult mouse (ICR) testis using RT-PCR and immunohistochemistry. The PCR revealed the presence of the identical products in the reactions for three LH subunit types. The expected product sizes for mouse Cgα and LH-β known as pituitary type were 224 bp and 503 bp, respectively. The testicular type

SUBMITTER: Kim HS 

PROVIDER: S-EPMC5651699 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature