Genome-wide engineering of an infectious clone of herpes simplex virus type 1 using synthetic genomics assembly methods.

ABSTRACT: Here, we present a transformational approach to genome engineering of herpes simplex virus type 1 (HSV-1), which has a large DNA genome, using synthetic genomics tools. We believe this method will enable more rapid and complex modifications of HSV-1 and other large DNA viruses than previous technologies, facilitating many useful applications. Yeast transformation-associated recombination was used to clone 11 fragments comprising the HSV-1 strain KOS 152 kb genome. Using overlapping sequences between the adjacent pieces, we assembled the fragments into a complete virus genome in yeast, transferred it into an Escherichia coli host, and reconstituted infectious virus following transfection into mammalian cells. The virus derived from this yeast-assembled genome, KOS^YA, repli

SUBMITTER: Oldfield LM 

PROVIDER: S-EPMC5651731 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature