Zymophore identification enables the discovery of novel phenylalanine ammonia lyase enzymes.

ABSTRACT: The suite of biological catalysts found in Nature has the potential to contribute immensely to scientific advancements, ranging from industrial biotechnology to innovations in bioenergy and medical intervention. The endeavour to obtain a catalyst of choice is, however, wrought with challenges. Herein we report the design of a structure-based annotation system for the identification of functionally similar enzymes from diverse sequence backgrounds. Focusing on an enzymatic activity with demonstrated synthetic and therapeutic relevance, five new phenylalanine ammonia lyase (PAL) enzymes were discovered and characterised with respect to their potential applications. The variation and novelty of various desirable traits seen in these previously uncharacterised enzymes demonstrates the importan

SUBMITTER: Weise NJ 

PROVIDER: S-EPMC5651878 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature