Analyzing homoeolog expression provides insights into the rediploidization event in gynogenetic hybrids of Carassius auratus red var. × Cyprinus carpio.

ABSTRACT: Rediploidization is considered to be a part of the evolutionary history of allotetraploids, and resulted in the emergence of novel epigenetic regulatory activities. To study the changing patterns of gene expression following the reduction of a genome by 50%, we used RNA-seq and quantitative real-time PCR (qPCR) to investigate total gene expression and homoeolog expression in three hybrids of a C. auratus red var. (2n = 100, ♀) (R) and C. carpio (2n = 100, ♂) (C) (i.e., F₁, F₁₈, and G₄) and their original parents. A comparison of homoeolog expression between G₄ and F₁₈ identified 7 genes (0.22%) that exhibited novel R/C homoeolog expression patterns in G₄, while 4 genes (0.12%) were affected by R/C homoeolog silencing. We det

SUBMITTER: Ren L 

PROVIDER: S-EPMC5651915 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature