Effects of targeting SLC1A5 on inhibiting gastric cancer growth and tumor development in vitro and in vivo.

ABSTRACT:

Aims

To investigate the oncogenic effects of SLC1A5 on gastric cancer development in vitro and in vivo.

Methods

The expression level of SLC1A5 was detected in 70 gastric cancer paraffin-embedded tissues by immunohistochemistry and also was detected in gastric cancer cell lines by qRT-PCR and western blotting analysis. The effects of knockdown SLC1A5 were analyzed on cell proliferation, cell cycle, the ability of cell migration and invasion and growth signaling pathway in vitro. By using subcutaneous xenograft mouse, the importance of SLC1A5 expression was assessed for both successful engraftment and growth of gastric cancer cells in vivo.

Results

SLC1A5 was up-regulated in gastric cancer tissues and was correlated with malignant features su

SUBMITTER: Lu J 

PROVIDER: S-EPMC5652719 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature