Identification of Osr2 Transcriptional Target Genes in Palate Development.

ABSTRACT: Previous studies have identified the odd-skipped related 2 (Osr2) transcription factor as a key intrinsic regulator of palatal shelf growth and morphogenesis. However, little is known about the molecular program acting downstream of Osr2 in the regulation of palatogenesis. In this study, we isolated palatal mesenchyme cells from embryonic day 12.5 (E12.5) and E13.5 Osr2^RFP/+ and Osr2^RFP/- mutant mouse embryos and performed whole transcriptome RNA sequencing analyses. Differential expression analysis of the RNA sequencing datasets revealed that expression of 70 genes was upregulated and expression of 61 genes was downregulated by >1.5-fold at both E12.5 and E13.5 in the Osr2^RFP/- palatal mesenchyme cells, in comparison with Osr2^RFP/+ littermates.

SUBMITTER: Fu X 

PROVIDER: S-EPMC5652858 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature