Direct screening of enzyme activity using infrared matrix-assisted laser desorption electrospray ionization.

ABSTRACT:

Rationale

High-throughput screening (HTS) is a critical step in the drug discovery process. However, most mass spectrometry (MS)-based HTS methods require sample cleanup steps prior to analysis. In this work we present the utility of infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) for monitoring an enzymatic reaction directly from a biological buffer system with no sample cleanup and at high throughput.

Methods

IR-MALDESI was used to directly analyze reaction mixtures from a well plate at different time points after reaction initiation. The percent conversion of precursors to products was used to screen the enzyme activity. The reaction was performed with two different concentrations of precursors and enzyme in order to assess the dynamic range

SUBMITTER: Nazari M 

PROVIDER: S-EPMC5653407 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature