Effects of Autologous Platelet-Rich Plasma on Regeneration of Damaged Endometrium in Female Rats.

ABSTRACT:

Purpose

To investigate whether autologous platelet-rich plasma (PRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced damage.

Materials and methods

Sixty female Sprague-Dawley rats were randomly assigned into three groups: control group, ethanol group, and PRP-treated group (administration of 0.25 mL of PRP into both uterine cavities 72 hours after ethanol injection). After 15 days of endometrial damage, all the animals were sacrificed during the estrous cycle, and samples were taken from the mid-uterine horn. Functional and structural recovery of the endometrium was analyzed by hematoxylin-eosin (H&E) and Masson trichrome (MT) staining, real-time polymerase chain reaction (PCR) assay, and immuno-histochemical (IHC) analyses.<

SUBMITTER: Jang HY 

PROVIDER: S-EPMC5653485 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature