Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells.

ABSTRACT: Understanding the biomolecular interactions between graphene and human immune cells is a prerequisite for its utilization as a diagnostic or therapeutic tool. To characterize the complex interactions between graphene and immune cells, we propose an integrative analytical pipeline encompassing the evaluation of molecular and cellular parameters. Herein, we use single-cell mass cytometry to dissect the effects of graphene oxide (GO) and GO functionalized with amino groups (GONH₂) on 15 immune cell populations, interrogating 30 markers at the single-cell level. Next, the integration of single-cell mass cytometry with genome-wide transcriptome analysis shows that the amine groups reduce the perturbations caused by GO on cell metabolism and increase biocompatibility. Moreover, GONH

SUBMITTER: Orecchioni M 

PROVIDER: S-EPMC5653675 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature