Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty.

ABSTRACT:

Background

common use of sevoflurane in congenital defects during repeated surgeries may have detrimental effects on spermatogenesis after puberty.

Objective

This study investigated sevoflurane effects on spermatogenesis process in male mature mice after exposure in prepubertal time.

Materials and methods

24 neonatal NMRI male mice were randomly classified in three groups. Experimental 1 and 2 groups (exposure to 1 minimum alveolar concentration (MAC) and 2 MAC sevoflurane, respectively in 2 lit/min oxygen (O₂) for 7 days (30 min, daily) and control. All groups were sacrificed after 2 months. Histological assessment, immunohistochemistry and apoptosis process was done. Bax and Bcl₂ expression was evaluated in the testicular tissue by real time Po

SUBMITTER: Nezhad Sistani M 

PROVIDER: S-EPMC5653908 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature