Editing inducer elements increases A-to-I editing efficiency in the mammalian transcriptome.

ABSTRACT:

Background

Adenosine to inosine (A-to-I) RNA editing has been shown to be an essential event that plays a significant role in neuronal function, as well as innate immunity, in mammals. It requires a structure that is largely double-stranded for catalysis but little is known about what determines editing efficiency and specificity in vivo. We have previously shown that some editing sites require adjacent long stem loop structures acting as editing inducer elements (EIEs) for efficient editing.

Results

The glutamate receptor subunit A2 is edited at the Q/R site in almost 100% of all transcripts. We show that efficient editing at the Q/R site requires an EIE in the downstream intron, separated by an internal loop. Also, other efficiently edited sites are flanked by conserved, h

SUBMITTER: Daniel C 

PROVIDER: S-EPMC5654063 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature