Use of a bioinformatic-assisted primer design strategy to establish a new nested PCR-based method for Cryptosporidium.

ABSTRACT:

Background

The accurate tracking of Cryptosporidium in faecal, water and/or soil samples in water catchment areas is central to developing strategies to manage the potential risk of cryptosporidiosis transmission to humans. Various PCR assays are used for this purpose. Although some assays achieve specific amplification from Cryptosporidium DNA in animal faecal samples, some do not. Indeed, we have observed non-specificity of some oligonucleotide primers in the small subunit of nuclear ribosomal RNA gene (SSU), which has presented an obstacle to the identification and classification of Cryptosporidium species and genotypes (taxa) from faecal samples.

Results

Using a novel bioinformatic approach, we explored all available Cryptosporidium genome sequences for new and diagnosti

SUBMITTER: Koehler AV 

PROVIDER: S-EPMC5654123 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature