Identification of CEA-interacting proteins in colon cancer cells and their changes in expression after irradiation.

ABSTRACT:

Purpose

The serum carcinoembryonic antigen (CEA) level has been recognized as a prognostic factor in colorectal cancer, and associated with response of rectal cancer to radiotherapy. This study aimed to identify CEA-interacting proteins in colon cancer cells and observe post-irradiation changes in their expression.

Materials and methods

CEA expression in colon cancer cells was examined by Western blot analysis. Using an anti-CEA antibody or IgG as a negative control, immunoprecipitation was performed in colon cancer cell lysates. CEA and IgG immunoprecipitates were used for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Proteins identified in the CEA immunoprecipitates but not in the IgG immunoprecipitates were selected as CEA-interacting proteins. After

SUBMITTER: Yoo BC 

PROVIDER: S-EPMC5654139 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature