Silencing HOXD10 by promoter region hypermethylation activates ERK signaling in hepatocellular carcinoma.

ABSTRACT:

Background

Hepatocellular carcinoma is the fifth most common malignancy and the third leading cause of cancer-related death worldwide. Dysregulation of HomeoboxD10 (HOXD10) was found to suppress or promote cancer progression in different cancer types. The function and regulation of HOXD10 remain unclear in human hepatocellular carcinoma (HCC).

Methods

Primary HCC samples (117), normal liver tissue samples (15), and 13 HCC cell lines (SNU182, SNU449, HBXF344, SMMC7721, Huh7, HepG2, LM3, PLC/PRF/5, BEL7402, SNU387, SNU475, QGY7703, and Huh1) were included in this study. Methylation-specific PCR, flow cytometry, western blot, transwell, siRNA, and chromatin immunoprecipitation assays were employed.

Results

HOXD10 was methylated in 76.9% (90/117) of human primary HCC samp

SUBMITTER: Guo Y 

PROVIDER: S-EPMC5654145 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature