Effect of cytomegalovirus and Epstein-Barr virus replication on intestinal mucosal gene expression and microbiome composition of HIV-infected and uninfected individuals.

ABSTRACT:

Background

HIV-infection is associated with dramatic changes in the intestinal mucosa. The impact of other viral pathogens is unclear.

Methods

One hundred and eight (108) biopsies from left and right colon (n = 79) and terminal ileum (n = 29) were collected from 19 HIV-infected and 22 HIV-uninfected participants. Levels of cytomegalovirus (CMV) and Epstein-Barr virus (EBV) DNA were measured by droplet digital PCR. Mucosal gene expression was measured via multiplex-assay. Microbiome analysis was performed using bacterial 16S-rDNA-pyrosequencing. The effect of CMV and EBV replication on the microbiome composition and mRNA-expression of selected cytokines (IL-6, IFN-γ, IL-1β, CCL2, IL-8, and IFN-β1) was evaluated.

Results

Overall, CMV and EBV were detected in at least on

SUBMITTER: Gianella S 

PROVIDER: S-EPMC5654609 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature