An RNA polymerase II-driven Ebola virus minigenome system as an advanced tool for antiviral drug screening.

ABSTRACT: Ebola virus (EBOV) causes a severe disease in humans with the potential for significant international public health consequences. Currently, treatments are limited to experimental vaccines and therapeutics. Therefore, research into prophylaxis and antiviral strategies to combat EBOV infections is of utmost importance. The requirement for high containment laboratories to study EBOV infection is a limiting factor for conducting EBOV research. To overcome this issue, minigenome systems have been used as valuable tools to study EBOV replication and transcription mechanisms and to screen for antiviral compounds at biosafety level 2. The most commonly used EBOV minigenome system relies on the ectopic expression of the T7 RNA polymerase (T7), which can be limiting for certain cell types. We have

SUBMITTER: Nelson EV 

PROVIDER: S-EPMC5654650 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature