An optimized protocol for the generation and functional analysis of human mast cells from CD34⁺ enriched cell populations.

ABSTRACT: The culture of mast cells from human tissues such a cord blood, peripheral blood or bone marrow aspirates has advanced our understanding of human mast cells (huMC) degranulation, mediator production and response to pharmacologic agents. However, existing methods for huMC culture tend to be laborious and expensive. Combining technical approaches from several of these protocols, we designed a simplified and more cost effective approach to the culture of mast cells from human cell populations including peripheral blood and cryopreserved cells from lymphocytapheresis. On average, we reduced by 30-50 fold the amount of culture media compared to our previously reported method, while the total MC number generated by this method (2.46±0.63×10⁶ vs. 2.4±0.28×10⁶, respectively,

SUBMITTER: Yin Y 

PROVIDER: S-EPMC5654741 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature